Dilute cells as necessary for appropriate input into the 10X Chromium chip. Proceed to: Chromium Single Cell 3' Reagent Kits v3 User Guide with Feature Barcoding technology for Cell Surface Protein (CG000206 Rev D) for TotalSeq-B reagents . OR
cytometry and used as input for the 10x Genomics Chromium Single Cell Immune Profiling Solution with Feature Barcoding technology. Libraries were prepared to characterize gene expression, cell surface protein expression, paired TCR sequences, and TCR-pMHC binding in each single cell.
10x Genomics Cloud Analysis enables you to process your single cell gene expression data through a simple web interface, leveraging an optimized, scalable infrastructure for fast results. Users can set up and run Cell Ranger pipelines through Cloud Analysis. Cell Ranger is a set of analysis pipelines that will automatically generate expression profiles for each cell and identify clusters of ...
As you can see, a key input in this command is the feature_reference.csv which, according to 10X, "declares the set of Feature Barcoding reagents in use in the experiment. For each unique Feature Barcode used, this file declares a feature name and identifier, the unique Feature Barcode sequence associated with this reagent, and a pattern ...
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Automated User Guide / Protocol 3 Sequence The resulting 10x barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for immune and transcriptional profiling of thousands of individual cells. Resources Sequencer Compatibility List 4 Analyze your data
Save for later. ORLANDO (Florida) - 10x Genomics this week previewed three new single-cell assays that it plans to launch this year for its Chromium Controller platform: single-cell CNV, single-cell ATAC-seq, and single-cell feature barcoding. The company showcased the assays at the Advances in Genome Biology and Technology meeting held here.
Purchase the Dual Index Kit TT Set A, the Dual Index Kit NT Set A, and the 3' Feature Barcode Kit; Use these Dual Index plates and Feature Barcode kit together with your existing 3'v3.1 chips, gel beads, and reagents, following the User Guide for 3' v3.1 Dual Index Gene Expression and CRISPR Screening libraries.
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ies were generated as described in our User Guide (Document CG000185), with each library aiming to recover either ~5,000 (PBMCs) or ~10,000 cells (MALT) (Figure 1). Sequencing of both Gene Expression and Feature Barcoding libraries was performed on an Illumina NovaSeq 6000 with NovaSeq software v1.2 using single-end sequencing, with a 28 bp (R1),
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